en باکتری شناسی Bacteriology پژوهشي Research <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Introduction:</span></span></b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"> <i>Staphylococcus aureus</i> is the most common bacterium causing surgical site infections (SSIs). The biofilm formation of <i>S. aureus</i> leads to increased survival and bacterial persistence. This study aimed to evaluate the frequency of biofilm formation and the presence of <i>icaD, icaA, fnbA, clfA</i>, and <i>cna</i> genes in <i>S. aureus</i> from SSIs isolated from patients in two hospitals in southwest Iran.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:4.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Methods: </span></span></b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">This cross-sectional study included 77 <i>S. aureus</i> isolates from SSIs. The ability of <i>S. aureus</i> for biofilm formation was assessed by the Congo Red Agar method. The prevalence of <i>icaD, icaA, clfA, fnbA, cna</i>, and <i>mecA</i> genes was determined by PCR. Data were analyzed using SPSS software (version 20) through chi-square test and Fisher&#39;s exact tests.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:4.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Results:</span></span></b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"> Biofilm formation was observed in 55 isolates (71.4%), including 30 (54.5%) strong and 25 (45.5%) weak biofilm producers&rsquo; isolates. The ability to form biofilm was higher among MRSA isolates (83.7%) compared to MSSA isolates (50%). The frequency of the <i>icaD, fnbA, clfA, cna, </i>and <i>icaA</i> genes was 62.3%, 42.9%, 28.6%, 20.8%, and 19.5%, respectively. There was a significant association between the presence of<i> fnbA </i>(P=0.000),<i> clfA </i>(P=0.000),<i> icaD </i>(P=0.02) genes and biofilm formation; however, it was not observed for <i>icaA </i>(P=0.054), and <i>cna</i> (P=0.132) genes.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:4.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Conclusions:</span></span></b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"> Our findings reinforce the role of Biofilm-related genes (fnbA, clfA, icaD) and biofilm formation. Given the role of genes in biofilm formation, we can pursue the development of scientific solutions to control biofilms, which ultimately lead to improved public health.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:9.0pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:4.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Key words:</span></span></b><span style="font-size:10.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"> Polymerase Chain Reaction, <i>Staphylococcus aureus</i>, Surgical Wound Infection</span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:114%"><span style="font-family:Calibri"><span style="font-size:12.0000pt"><span style="font-family:'Times New Roman'"><span style="line-height:114%"></span></span></span></span></span></span></span><br> &nbsp; Polymerase Chain Reaction, Staphylococcus aureus, Surgical Wound Infection 0 0 http://jsurgery.bums.ac.ir/browse.php?a_code=A-10-523-1&slc_lang=en&sid=1 zaker saeedinegad saeedizaker@yahoo.com 100319475328460012384 100319475328460012384 No Assistant Professor of Infectious Disease, Department of Internal Medicine, School of Medicine, Shahid Beheshti Hospital, Yasuj University of Medical Sciences, Iran. FARZAD mazloomirad kmazlumirad@gmail.com 100319475328460012385 100319475328460012385 Yes Student Research Committee, Yasuj University of Medical Sciences, Yasuj. Iran