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Moosa Javdani * 
, Mohammad Mahdi Bemanizadeh , Pegah Khosravian , Mohammad Javad Kouhian , Elham Moghtadaei Khorasgani , Mohammad Salimi Beni
, Mohammad Mahdi Bemanizadeh , Pegah Khosravian , Mohammad Javad Kouhian , Elham Moghtadaei Khorasgani , Mohammad Salimi Beni
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Abstract: (22 Views)
Introduction: Postoperative peritoneal adhesions remain a major clinical challenge following abdominal surgery, frequently leading to intestinal obstruction, chronic pain, and infertility. Oxidative stress and inflammation are known to play central roles in the pathogenesis of adhesion formation. This study evaluated the preventive effects of selenium nanoparticles and coenzyme Q10, administered individually and in combination, in a rat model of peritoneal adhesions.
Methods: This was a randomized, controlled experimental study. Sixty male Wistar rats were divided into six groups (n = 10 per group; 5 rats per time point): Sham, Control, Solvent, SeNPs (0.2 mg/kg), CoQ10 (10 mg/kg), and Mix (SeNPs + CoQ10). Adhesions were induced using the cecal scratch model. Treatments were administered intraperitoneally for four consecutive days. On days 7 and 14, adhesion severity was assessed macroscopically, and tissue samples were collected for histopathological evaluation of inflammation, vascularization, and fibroplasia. Antioxidant enzyme activities (SOD, GPx), malondialdehyde levels, and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) were measured. Statistical analysis was performed using SPSS, version 27.0 and one-way ANOVA, followed by Tukey's post hoc test was used for normally distributed data, whereas the Kruskal–Wallis test followed by the Mann–Whitney U test with Bonferroni correction was used for non-normally distributed data.
Results: The combination therapy significantly reduced adhesion severity compared with the control and monotherapy groups on both days 7 and 14 (P < 0.05). Histopathological analysis revealed that the combination group exhibited the highest fibroplasia scores (least fibrosis), which were comparable to those of the sham group. Biochemical analyses showed that the combination treatment normalized malondialdehyde levels, restored SOD and GPx activities, and significantly suppressed TNF-α, IL-1β, and IL-6 levels (P < 0.05), reaching levels indistinguishable from those of the sham group.
Conclusions: Co-administration of selenium nanoparticles and coenzyme Q10 exerts synergistic protection against peritoneal adhesions by enhancing antioxidant defenses and suppressing pro-inflammatory cytokines. This combination represents a promising dual-agent strategy for preventing postoperative peritoneal adhesions.
Methods: This was a randomized, controlled experimental study. Sixty male Wistar rats were divided into six groups (n = 10 per group; 5 rats per time point): Sham, Control, Solvent, SeNPs (0.2 mg/kg), CoQ10 (10 mg/kg), and Mix (SeNPs + CoQ10). Adhesions were induced using the cecal scratch model. Treatments were administered intraperitoneally for four consecutive days. On days 7 and 14, adhesion severity was assessed macroscopically, and tissue samples were collected for histopathological evaluation of inflammation, vascularization, and fibroplasia. Antioxidant enzyme activities (SOD, GPx), malondialdehyde levels, and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) were measured. Statistical analysis was performed using SPSS, version 27.0 and one-way ANOVA, followed by Tukey's post hoc test was used for normally distributed data, whereas the Kruskal–Wallis test followed by the Mann–Whitney U test with Bonferroni correction was used for non-normally distributed data.
Results: The combination therapy significantly reduced adhesion severity compared with the control and monotherapy groups on both days 7 and 14 (P < 0.05). Histopathological analysis revealed that the combination group exhibited the highest fibroplasia scores (least fibrosis), which were comparable to those of the sham group. Biochemical analyses showed that the combination treatment normalized malondialdehyde levels, restored SOD and GPx activities, and significantly suppressed TNF-α, IL-1β, and IL-6 levels (P < 0.05), reaching levels indistinguishable from those of the sham group.
Conclusions: Co-administration of selenium nanoparticles and coenzyme Q10 exerts synergistic protection against peritoneal adhesions by enhancing antioxidant defenses and suppressing pro-inflammatory cytokines. This combination represents a promising dual-agent strategy for preventing postoperative peritoneal adhesions.
Type of Study: Research |
Subject:
General Surgery
Received: 2026/07/10 | Accepted: 2026/10/10 | ePublished ahead of print: 2026/11/3
Received: 2026/07/10 | Accepted: 2026/10/10 | ePublished ahead of print: 2026/11/3
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