Volume 14, Issue 2 (6-2026)
J Surg Trauma 2026, 14(2): 86-92 |
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Saeedi Nejad Z, Mazloomirad F. Staphylococcus Aureus Isolated from Surgical Site Infections: Evaluation of Biofilm and Biofilm-Related Genes. J Surg Trauma 2026; 14 (2) :86-92
URL: http://jsurgery.bums.ac.ir/article-1-517-en.html
URL: http://jsurgery.bums.ac.ir/article-1-517-en.html
Student Research Committee, Yasuj University of Medical Sciences, Yasuj. Iran
Abstract: (569 Views)
Introduction: Staphylococcus aureus is the most common bacterium causing surgical site infections (SSIs). The biofilm formation of S. aureus leads to increased survival and bacterial persistence. This study aimed to evaluate the frequency of biofilm formation and the presence of icaD, icaA, fnbA, clfA, and cna genes in S. aureus from SSIs isolated from patients in two hospitals in southwest Iran.
Methods: This cross-sectional study included 77 S. aureus isolates from SSIs. The ability of S. aureus for biofilm formation was assessed by the Congo Red Agar method. The prevalence of icaD, icaA, clfA, fnbA, cna, and mecA genes was determined by PCR. Data were analyzed using SPSS software (version 20) through chi-square test and Fisher's exact tests.
Results: Biofilm formation was observed in 55 isolates (71.4%), including 30 (54.5%) strong and 25 (45.5%) weak biofilm producers’ isolates. The ability to form biofilm was higher among MRSA isolates (83.7%) compared to MSSA isolates (50%). The frequency of the icaD, fnbA, clfA, cna, and icaA genes was 62.3%, 42.9%, 28.6%, 20.8%, and 19.5%, respectively. There was a significant association between the presence of fnbA (P=0.000), clfA (P=0.000), icaD (P=0.02) genes and biofilm formation; however, it was not observed for icaA (P=0.054), and cna (P=0.132) genes.
Conclusions: Our findings reinforce the role of Biofilm-related genes (fnbA, clfA, icaD) and biofilm formation. Given the role of genes in biofilm formation, we can pursue the development of scientific solutions to control biofilms, which ultimately lead to improved public health.
Key words: Polymerase Chain Reaction, Staphylococcus aureus, Surgical Wound Infection
Methods: This cross-sectional study included 77 S. aureus isolates from SSIs. The ability of S. aureus for biofilm formation was assessed by the Congo Red Agar method. The prevalence of icaD, icaA, clfA, fnbA, cna, and mecA genes was determined by PCR. Data were analyzed using SPSS software (version 20) through chi-square test and Fisher's exact tests.
Results: Biofilm formation was observed in 55 isolates (71.4%), including 30 (54.5%) strong and 25 (45.5%) weak biofilm producers’ isolates. The ability to form biofilm was higher among MRSA isolates (83.7%) compared to MSSA isolates (50%). The frequency of the icaD, fnbA, clfA, cna, and icaA genes was 62.3%, 42.9%, 28.6%, 20.8%, and 19.5%, respectively. There was a significant association between the presence of fnbA (P=0.000), clfA (P=0.000), icaD (P=0.02) genes and biofilm formation; however, it was not observed for icaA (P=0.054), and cna (P=0.132) genes.
Conclusions: Our findings reinforce the role of Biofilm-related genes (fnbA, clfA, icaD) and biofilm formation. Given the role of genes in biofilm formation, we can pursue the development of scientific solutions to control biofilms, which ultimately lead to improved public health.
Key words: Polymerase Chain Reaction, Staphylococcus aureus, Surgical Wound Infection
Type of Study: Research |
Subject:
Bacteriology
Received: 2025/10/20 | Accepted: 2026/02/16 | ePublished ahead of print: 2026/05/6 | Published: 2026/06/6
Received: 2025/10/20 | Accepted: 2026/02/16 | ePublished ahead of print: 2026/05/6 | Published: 2026/06/6
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